Goat anti-NEDD1 Antibody

Goat anti-NEDD1 Antibody
Principle Name: NEDD1
Official Symbol: NEDD1
All Name and Symbols: NEDD1; neural precursor cell expressed, developmentally down-regulated 1
Accession Number(s): NP_690869.1
Peptide Sequence: NQTRNSEKFEKPEN, is from internal region
Human Gene ID(s): 121441
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 75kDa band observed in lysates of cell line HeLa (calculated MW of 72.0kDa according to NP_690869.1). Recommended concentration: 1-3ug/ml.
An additional band was also consistently observed at 37kDa. This band was successfully blocke
Reference(s): Haren L, Remy MH, Bazin I, Callebaut I, Wright M, Merdes A. NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly. J Cell Biol. 2006 Feb 13; 172(4):505-15. Epub 2006 Feb 6. .PMID: 16461362