Goat anti-EEF1E1 / AIMP3 / p18 Antibody

Pep ELISA, WB

Goat anti-EEF1E1 / AIMP3 / p18 Antibody
Principle Name: EEF1E1 / AIMP3 / p18
Official Symbol: EEF1E1
All Name and Symbols: EEF1E1; eukaryotic translation elongation factor 1 epsilon 1; AIMP3; P18; ARS-interacting multifunctional protein 3; aminoacyl tRNA synthetase complex-interacting multifunctional protein 3; eukaryotic translation elongation factor 1 epsilon-1
Accession Number(s): NP_004271.1; NP_001129122.1
Peptide Sequence: SKGNKYSAQGERQ, is from internal region (near N Terminus)
Human Gene ID(s): 9521
Important Comments: This antibody is expected to recognize both reported isoforms (NP_004271.1; NP_001129122.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 19kDa band observed in lysates of cell lines HepG2 and K562 (calculated MW of 19.8kDa according to NP_004271.1) and in Pig Testis lysates (calculated MW of 19.8kDa according to NP_001230474.1). Recommended concentration: 0.1-0.3ug/ml
Reference(s): Kwon NH, Kang T, Lee JY, Kim HH, Kim HR, Hong J, Oh YS, Han JM, Ku MJ, Lee SY, Kim S. Dual role of methionyl-tRNA synthetase in the regulation of translation and tumor suppressor activity of aminoacyl-tRNA synthetase-interacting multifunctional protein-3. Proc Natl Acad Sci U S A. 2011 Dec 6; 108(49):19635-40.PMID: 22106287