Vergleich

Neurofilament Medium (Neurofilament Medium Polypeptide, NF-M, 160kD Neurofilament Protein, Neurofilament 3, Neurofilament Triplet M Protein, Nefm, Nef3, Nfm)

ArtNr USB-215394
Hersteller United States Biological
Menge 100 ul
Kategorie
Typ Antibody Monoclonal
Format Lyophilized
Applikationen WB, IC
Clon 3H11
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Chicken (Gallus gallus domesticus), Pig (Porcine, Sus scrofa domesticus), Cat (Feline, Felis catus), Cattle (Bovine)
Host Mouse
Isotype IgG1
Purity Purified
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Mab
Manufacturer - Category
Antibodies / Antibodies-Neurofilament
Shipping Temperature
Blue Ice
Storage Conditions
-20°C
Grade
Purified
Form
Supplied as a lyophilized powder in 5% trehalose. Reconstitute with sterile ddH2O.
EU Commodity Code
38221010
Immunogen
Raised against a recombinant fusion protein containing the extreme C-terminal of rat NF-M, expressed in E. coli. Epitope: localized to within the last 56aa at the extreme C-terminal of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species.
Specificity
Recognizes rat Neurofilament Medium. Species Crossreactivity: Huamn, mouse, feline, bovine, porcine, chicken
Description
Neurofilaments are composed of three intermediate filament proteins: light (~68kD), medium (~160kD) and heavy (~200kD), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170kD, with some variation in different species.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
Immunocytochemistry: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ul
Lieferbar: In stock
lieferbar

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