Neurofilament Medium (Neurofilament Medium Polypeptide, NF-M, 160kD Neurofilament Protein, Neurofilament 3, Neurofilament Triplet M Protein, Nefm, Nef3, Nfm)

Antibodies / Antibodies-Neurofilament

-20°C

Supplied as a lyophilized powder in 5% trehalose. Reconstitute with sterile ddH2O.

Raised against a recombinant fusion protein containing the extreme C-terminal of rat NF-M, expressed in E. coli. Epitope: localized to within the last 56aa at the extreme C-terminal of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species.

Neurofilaments are composed of three intermediate filament proteins: light (~68kD), medium (~160kD) and heavy (~200kD), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170kD, with some variation in different species.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
Immunocytochemistry: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.