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Anti-Human IL-10 Antibody

Anti-Human IL-10 Antibody

ArtNr	ABC-ABG10188-U050
Hersteller	Abcepta
Menge	50 ug
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Applikationen	WB, IHC, ELISA
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Lieferbar
Application	WB, IHC, E
Clonality	Polyclonal
Reactivity	H
Preparation	Produced from sera of rabbits pre-immunized with highly pure (> 98%) recombinant hIL-10. Anti-Human IL-10 specific antibody was purified by affinity chromatography employing immobilized hIL-10 matrix.
WesternBlot	To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µ g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich	To detect hIL-10 by sandwich ELISA (using 100 μ l/well antibody solution) a concentration of 0.5 - 2.0 μ g/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IL-10 (60-010BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-10.
Immunohistochemistry	This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Neutralization	To yield one-half maximal inhibition [ND₅₀] of the biological activity of hIL-10 (1.0 ng/ml), a concentration of 1.2-1.8 µ g/ml of this antibody is required.
Formulation	A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution	Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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ABC-ABG10188-U050-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 50 ug

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Lieferung vsl. bis 02.10.2025

Express-Lieferung: 22 Tage sparen

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