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Anti-Human MIA-2 Antibody

ArtNr ABC-ABG10389-U050
Hersteller Abcepta
Menge 50 ug
Kategorie
Typ Antibody Primary
Applikationen WB, IHC, ELISA
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Lieferbar
Application
WB, IHC, E
Clonality
Polyclonal
Reactivity
H
Preparation
Produced from sera of rabbits pre-immunized with highly pure (> 98%) recombinant hMIA-2 (human MIA-2). Anti-Human MIA-2 specific antibody was purified by affinity chromatography employing immobilized hMIA-2 matrix.
WesternBlot
To detect hMIA-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich
To detect hMIA-2 by sandwich ELISA (using 100μ l/well antibody solution) a concentration of 0.5 - 2.0 μ g/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human MIA-2 (60-220BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2.
Immunohistochemistry
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 5.0 μ g/ml with an overnight incubation at 4° C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary.
Formulation
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 ug
Lieferbar: In stock
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