Anti-CIITA Picoband Antibody

P33076

Polyclonal

H, M, Rat

WB

CIITA was detected in paraffin-embedded sections of mouse spleen tissues using rabbit anti- CIITA Antigen Affinity purified polyclonal antibody (Catalog # ABO12681) at 1 μg/mL. The immunohistochemical section was developed using SABC method .

0.5-1 µg/ml

IHC

IHC

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

|immunology|adaptive immunity|mhc|class ii|
epigenetics and nuclear signaling|transcription|other factors

MHC class II transactivator

Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro). .

E.coli-derived human CIITA recombinant protein (Position: E945-R1130). Human CIITA shares 84.4% amino acid (aa) sequence identity with mouse CIITA.

No cross reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.