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ME2 Antibody (C-term)

ME2 Antibody (C-term)

ArtNr	ABC-AM8621b
Hersteller	Abcepta
Menge	200 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applikationen	WB, IHC-P
Specific against	other
Host	Mouse
Isotype	IgG1 kappa
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	ME2, mitochondrial, NAD-dependent malic enzyme, Malic enzyme 2, NAD-ME, 1.1.1.38
Lieferbar
Primary Accession	P23368
Antigen Type	Synthesized Peptide
Application	WB, IHC-P
Bio References	Loeber G., et al.J. Biol. Chem. 266:3016-3021(1991). Yanaihara N., et al.Genomics 72:169-179(2001). Ota T., et al.Nat. Genet. 36:40-45(2004). Nusbaum C., et al.Nature 437:551-555(2005). Mural R.J., et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Clonality	monoclonal
Clone/Animal Names	970CT2.1.1.2
Gene ID	4200
Gene Name	ME2
Subtitle	Purified Mouse Monoclonal Antibody (Mab)
Reactivity	H, M
Legend image 1	All lanes : Anti-ME2 Antibody (C-term) at 1:4000 dilution Lane 1: HL-60 whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:4000
Legend image 2	AM8621b staining ME2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 2	IHC-P
Dilution image 2	1:25
Isotype	IgG1, k
Calculated MW	65444
Antigen Source	HUMAN
Target/Specificity	This ME2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 522-556 amino acids from the C-terminal region of human ME2.

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Menge: 200 ul

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