Comparison

ME2 Antibody (C-term)

Item no. ABC-AM8621b
Manufacturer Abcepta
Amount 200 ul
Category
Type Antibody Primary
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applications WB, IHC-P
Specific against other
Host Mouse
Isotype IgG1 kappa
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products ME2, mitochondrial, NAD-dependent malic enzyme, Malic enzyme 2, NAD-ME, 1.1.1.38
Available
Primary Accession
P23368
Antigen Type
Synthesized Peptide
Application
WB, IHC-P
Bio References
Loeber G., et al.J. Biol. Chem. 266:3016-3021(1991).
Yanaihara N., et al.Genomics 72:169-179(2001).
Ota T., et al.Nat. Genet. 36:40-45(2004).
Nusbaum C., et al.Nature 437:551-555(2005).
Mural R.J., et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Clonality
monoclonal
Clone/Animal Names
970CT2.1.1.2
Gene ID
4200
Gene Name
ME2
Subtitle
Purified Mouse Monoclonal Antibody (Mab)
Reactivity
H, M
Legend image 1
All lanes : Anti-ME2 Antibody (C-term) at 1:4000 dilution
Lane 1: HL-60 whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lane 3: Jurkat whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 65 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1
WB
Dilution image 1
1:4000
Legend image 2
AM8621b staining ME2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 2
IHC-P
Dilution image 2
1:25
Isotype
IgG1, k
Calculated MW
65444
Antigen Source
HUMAN
Target/Specificity
This ME2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 522-556 amino acids from the C-terminal region of human ME2.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 200 ul
Available: In stock
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