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GSDMC Antibody (Center)

GSDMC Antibody (Center)

ArtNr	ABC-AP10771c
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	GSDMC, MLZE, Gasdermin-C, Melanoma-derived leucine zipper-containing extranuclear factor
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Primary Accession	Q9BYG8
Antigen Type	Synthetic Peptide
Application	IHC-P, WB, FC
Bio References	Birnbaum, S., et al. Nat. Genet. 41(4):473-477(2009) Saeki, N., et al. Genes Chromosomes Cancer 48(3):261-271(2009) Tamura, M., et al. Genomics 89(5):618-629(2007) Watabe, K., et al. Jpn. J. Cancer Res. 92(2):140-151(2001)
Clonality	Polyclonal
Clone/Animal Names	RB27043
Gene ID	56169
Gene Name	GSDMC {ECO:0000303\|PubMed:17350798, ECO:0000312\|HGNC:HGNC:7151}
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	All lanes : Anti-GSDMC Antibody (Center) at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: MKN45 whole cell lysate Lane 3: SK-MEL-2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	GSDMC antibody (Center) (Cat. #AP10771c) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSDMC antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 2	IHC-P
Dilution image 2	1:50~100
Legend image 3	Overlay histogram showing U-2OS cells stained with AP10771c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP10771c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 3	FC
Dilution image 3	1:25
Other Accession	NP_113603.1
Isotype	Rabbit Ig
Calculated MW	57692
Antigen Region	218-246
Antigen Source	HUMAN
Target/Specificity	This GSDMC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human GSDMC.
NCBI Accession	NP_113603.1

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ABC-AP10771c-datasheet.pdf

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Menge: 400 ul

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