IFITM5 Antibody (Center)

Synthetic Peptide

Plays a role in bone mineralization (By similarity).

Polyclonal

387733

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-IFITM5 Antibody (Center) at 1:2000 dilution
Lane 1: PC-3 whole cell lysate
Lane 2: SW480 whole cell lysate
Lane 3: HT-29 whole cell lysate
Lane 4: Mouse spleen lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 14 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

WB

Anti-IFITM5 Antibody (Center) at 1:2000 dilution + HT-29 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 14 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

IHC-P-Leica

Overlay histogram showing A431 cells stained with AP11058C(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

Rabbit IgG

55-83

This IFITM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids of human IFITM5.