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GNL3 Antibody (N-term)

GNL3 Antibody (N-term)

ArtNr	ABC-AP21197a-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IF
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	GNL3, Guanine nucleotide-binding protein-like 3, Nucleolar GTP-binding protein 3, Nucleostemin, NS, E2IG3, NNP47, Novel nucleolar protein 47, E2-induced gene 3 protein
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Primary Accession	Q9BVP2
Application	WB, IF
Bio Background	May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).
Bio References	Charpentier A.H., et al.Cancer Res. 60:5977-5983(2000). Han C., et al.Int. J. Mol. Med. 16:205-213(2005). Ota T., et al.Nat. Genet. 36:40-45(2004). Muzny D.M., et al.Nature 440:1194-1198(2006). Andersen J.S., et al.Curr. Biol. 12:1-11(2002).
Clonality	polyclonal
Clone/Animal Names	RB51601
Gene ID	26354
Gene Name	GNL3
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human cervical epithelial adenocarcinoma cell line) cells labeling GNL3 with AP21197a at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing both nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).
Type image 1	IF
Dilution image 1	1:25
Legend image 2	All lanes : Anti-GNL3 Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: NCCIT whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 62 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Isotype	Rabbit Ig
Calculated MW	61993
Antigen Source	HUMAN
Target/Specificity	This GNL3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 91-124 amino acids from the N-terminal region of human GNL3.

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ABC-AP21197a-ev-datasheet.pdf

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Menge: 80 ul

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Lieferung vsl. bis 04.09.2025