Cyclophilin B Antibody

P23284

WB, FC, IHC-P

Spik G., et al.J. Biol. Chem. 266:10735-10738(1991).
Ota T., et al.Nat. Genet. 36:40-45(2004).
Ebert L., et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Zody M.C., et al.Nature 440:671-675(2006).
Mural R.J., et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

RB55967

PPIB

H, M, Rat

WB

All lanes : Anti-Cyclophilin B Antibody at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: A431 whole cell lysate
Lane 4: BA/F3 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
lane 6: PC-12 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 24 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

IHC-P

Overlay histogram showing Hela cells stained with AP22114a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22114a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

1:25

M

23743

This Cyclophilin B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 161-195 amino acids from the human region of human Cyclophilin B.