XBP1 Antibody (Center)

Synthetic Peptide

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor.

Polyclonal

7494

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of XBP1 Antibody (Center) (Cat. #AP5088c) in Ramos cell line lysates (35ug/lane).XBP1 (arrow) was detected using the purified Pab.

1:1000

WB

XBP1 Antibody (Center) (Cat. #AP5088c) IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

1:50~100

FC

Q9R1S4; O35426; Q3SZZ2

Rabbit Ig

62-91

This XBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the Central region of human XBP1.