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Methionyl tRNA Synthetase Antibody (C-term)

ArtNr ABC-AP7841b-ev
Hersteller Abcepta
Menge 80 ul
Kategorie
Typ Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen WB, IHC-P
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products MARS2, mitochondrial, Methionine--tRNA ligase, MtMetRS, Methionyl-tRNA synthetase 2, Mitochondrial methionyl-tRNA synthetase
Lieferbar
Primary Accession
Q96GW9
Antigen Type
Synthetic Peptide
Application
IHC-P, WB
Bio Background
Methionine-tRNA ligase (EC 6.1.1.10) catalyzes the chemical reaction:

ATP + L-methionine + tRNAMet <--> AMP + diphosphate + L-methionyl-tRNAMet

The 3 substrates of this enzyme are ATP, L-methionine, and tRNA(Met), whereas its 3 products are AMP, diphosphate, and L-methionyl-tRNA(Met).

This enzyme participates in 3 metabolic pathways: methionine metabolism, selenoamino acid metabolism, and aminoacyl-trna biosynthesis.
Bio References
Spencer, A.C., Biochemistry 43 (30), 9743-9754 (2004)
Clonality
Polyclonal
Clone/Animal Names
RB14925
Gene ID
92935
Gene Name
MARS2
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H, M
Legend image 1
Western blot analysis of anti-MARS2 Antibody (C-term) (AP7841b) in mouse testis tissue lysates (35ug/lane). MARS2(arrow) was detected using the purified Pab.
Type image 1
WB
Dilution image 1
1:1000
Legend image 2
Anti-MARS2 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 67 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MARS2 antibody (C-term) (Cat.#AP7841b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3
IHC-P
Dilution image 3
1:10~50
Isotype
Rabbit Ig
Calculated MW
66591
Antigen Region
564-593
Antigen Source
HUMAN
Target/Specificity
This Methionyl tRNA Synthetase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Methionyl tRNA Synthetase.
NCBI Accession
NP_612404.1

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 80 ul
Lieferbar: In stock
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