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AOC3 Antibody (Center)

AOC3 Antibody (Center)

ArtNr	ABC-AP8538c
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	HPAO, AOC3, VAP-1, SSAO, VAP1, Membrane primary amine oxidase, Copper amine oxidase, Vascular adhesion protein 1, Semicarbazide-sensitive amine oxidase
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Primary Accession	Q16853
Antigen Type	Synthetic Peptide
Application	WB, FC, IHC-P
Bio Background	Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity.
Bio References	Lalor, P.F., et.al., J. Immunol. 169 (2), 983-992 (2002) Salmi, M., et.al., Am. J. Pathol. 161 (6), 2255-2262 (2002)
Clonality	Polyclonal
Clone/Animal Names	RB22029
Gene ID	8639
Gene Name	AOC3
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of AOC3 Antibody (Center) (Cat. #AP8538c) in CEM cell line lysates (35ug/lane).AOC3 (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	All lanes : Anti-AOC3 Antibody (Center) at 1:2000 dilution Lane 1: human lung lysate Lane 2: human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	Formalin-fixed and paraffin-embedded human colon carcinoma with AOC3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Legend image 4	AP8538c staining AOC3 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 4	IHC-P
Dilution image 4	1:25
Legend image 5	Flow cytometric analysis of CEM cells using AOC3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 5	FC
Dilution image 5	1:10~50
Isotype	Rabbit Ig
Calculated MW	84622
Antigen Region	613-640
Antigen Source	HUMAN
Target/Specificity	This AOC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 613-640 amino acids from the Central region of human AOC3.
NCBI Accession	NP_001264660.1; NP_001264661.1; NP_003725.1

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Menge: 400 ul

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