CAD Antibody

O54788

CAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

Polyclonal

Dffb

Immunohistochemistry of T cadherin in mouse brain tissue with T cadherin Antibodyat 5 µg/mL.

Western blot analysis of SARM in Daudi cell lysate with SARM antibody at (A) 0.5 and (B) 1 µg/mL.

O54788; 13368

39449

CAD antibody was raised against a 16 amino acid peptide near the carboxy terminus of murine CAD.

The immunogen is located within the last 50 amino acids of CAD.

CAD antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

CAD antibody antibody can be used for detection of CAD by Western blot at 0.5 μ g/mL. A 40 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 5 μ g/mL.

CAD Antibody is supplied in PBS containing 0.02% sodium azide.