PKR Antibody

P19525

PKR Antibody: The interferon-inducible, double-stranded RNA (dsRNA)-dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

Polyclonal

EIF2AK2

Immunohistochemistry of Clusterin in mouse brain tissue with Clusterin Antibodyat 5 µg/mL.

AAP57628; 5610

62094

PKR antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human PKR.

The immunogen is located within the last 50 amino acids of PKR.

PKR antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

PKR antibody can be used for detection of PKR by Western blot at 0.5 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 20 μ g/mL.

PKR Antibody is supplied in PBS containing 0.02% sodium azide.