FIH Antibody

ArtNr	ABC-ASM10125-FITC-100
Hersteller	Abcepta
Menge	Inquiry
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	FITC
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Lieferbar
Primary Accession	Q9NWT6
Reactivity	H, M
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FIH Monoclonal Antibody, Clone fih162C (ASM10125). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (ASM10125) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-FIH Antibody. (C) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Immunohistochemistry analysis using Mouse Anti-FIH Monoclonal Antibody, Clone fih162C (ASM10125). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (ASM10125) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All positive.
Type image 2	IHC
Dilution image 2	1:100
Legend image 3	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FIH Monoclonal Antibody, Clone fih162C (ASM10125). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (ASM10125) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-FIH Antibody. (C) Composite.
Type image 3	ICC/IF
Dilution image 3	1:100
Legend image 4	Western Blot analysis of Human Cell lysates showing detection of FIH protein using Mouse Anti-FIH Monoclonal Antibody, Clone fih162c (ASM10125). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (ASM10125) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Type image 4	WB
Dilution image 4	1:100
Other Accession	NP_060372.2
Isotype	IgG1
Target/Specificity	Detects ~45kDa.
Immunogen	Full length human FIH expressed in E.coli BL21 (DE3) cells
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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ABC-ASM10125-FITC-100-datasheet.pdf

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