HCN1 Antibody

ArtNr	ABC-ASM10182-100
Hersteller	Abcepta
Menge	Inquiry
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Unconjugated
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Lieferbar
Primary Accession	Q9JKBO
Reactivity	H, M, Rat
Legend image 1	Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells.
Type image 1	IHC
Dilution image 1	1:1000
Legend image 2	Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Type image 3	IHC
Legend image 4	Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons.
Type image 4	IHC
Dilution image 4	1:1000
Legend image 5	Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Type image 5	IHC
Dilution image 5	1:1000
Other Accession	NP_445827.1
Isotype	IgG1
Target/Specificity	Detects ~100kDa. No cross-reactivity against HCN2.
Immunogen	Fusion protein amino acids 778-910 (C terminus) of rat HCN1
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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