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Anti-Swine IgG (H&L) (Alkaline Phosphatase Conjugated) Secondary Antibody

ArtNr ABC-ASR2373
Hersteller Abcepta
Menge 1 mg
Kategorie
Typ Antibody Secondary
Specific against other
Host Rabbit
Konjugat/Tag AP
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Lieferbar
Clonality
Polyclonal
Subtitle
Rabbit Polyclonal, Alkaline Phosphatase (Calf Intestine)
Legend image 1
Western Blot of Alkaline Phosphatase conjugated Rabbit anti-Swine IgG antibody.
Lane 1: Swine IgG.
Lane 2: none.
Load: 100 ng per lane.
Primary antibody: none.
Secondary antibody: Alkaline Phosphatase swine secondary antibody at 1:1, 000 for 60 min at RT.
Block: MB-070 for 30 min RT.
Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG.
Other band(s): none.
Type image 1
WB
Dilution image 1
1:500 - 1:2, 500
Immunogen
Swine IgG whole molecule
Concentration (mg/ml)
1.0 mg/mL by UV absorbance at 280 nm
Conjugate
Alkaline Phosphatase (Calf Intestine)
Application Note
ELISA 1:14, 000; Western Blot 1:500-1:2, 500; Immunochemistry 1:200-1:1, 000
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Target Species
Swine
Physical State
Liquid (sterile filtered)
Target Isotype
IgG (H&L)
Host Isotype
IgG
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% (w/v) Sodium Azide
Shipping Condition
Wet Ice
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Swine IgG, and Swine Serum.
Storage Condition
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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