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F(ab')2 Anti-MOUSE IgM (mu chain) (Peroxidase Conjugated) Secondary Antibody

Hersteller Abcepta
Kategorie
Typ Antibody Secondary
Specific against other
Menge 500 ug
Host Goat
ArtNr ABC-ASR2991
Konjugat/Tag HRP
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Bio Background
F(ab')2 Anti-Mouse IgM mu heavy chain generated in goat detects specifically Mouse IgM mu heavy chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Clonality
Polyclonal
Subtitle
Goat Polyclonal, Peroxidase (Horseradish)
Reactivity
M
Immunogen
F(ab' )2 Anti-Mouse IgM mu heavy chain was produced by repeated immunization with Mouse IgM mu heavy chain in goat.
Concentration (mg/ml)
1.0 mg/mL by UV absorbance at 280 nm
Conjugate
Peroxidase (Horseradish)
Application Note
ELISA 1:100, 000; Western Blot 1:2, 000-1:10, 000; Immunochemistry 1:500-1:2, 500
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Target Species
Mouse
Physical State
Lyophilized
Target Isotype
IgM µ chain
Host Isotype
IgG F(ab')2
Reconstitution Volume
500 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Shipping Condition
Ambient
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).   Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 500 ug
Lieferbar: In stock
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Lieferung vsl. bis 04.07.2024 

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