Comparison

F(ab')2 Anti-MOUSE IgM (mu chain) (Peroxidase Conjugated) Secondary Antibody

Manufacturer Abcepta
Category
Type Antibody Secondary
Specific against other
Amount 500 ug
Host Goat
Item no. ABC-ASR2991
Conjugate/Tag HRP
eClass 6.1 32160702
eClass 9.0 32160702
Available
Bio Background
F(ab')2 Anti-Mouse IgM mu heavy chain generated in goat detects specifically Mouse IgM mu heavy chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Clonality
Polyclonal
Subtitle
Goat Polyclonal, Peroxidase (Horseradish)
Reactivity
M
Immunogen
F(ab' )2 Anti-Mouse IgM mu heavy chain was produced by repeated immunization with Mouse IgM mu heavy chain in goat.
Concentration (mg/ml)
1.0 mg/mL by UV absorbance at 280 nm
Conjugate
Peroxidase (Horseradish)
Application Note
ELISA 1:100, 000; Western Blot 1:2, 000-1:10, 000; Immunochemistry 1:500-1:2, 500
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Target Species
Mouse
Physical State
Lyophilized
Target Isotype
IgM µ chain
Host Isotype
IgG F(ab')2
Reconstitution Volume
500 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Shipping Condition
Ambient
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).   Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 500 ug
Available: In stock
available

Delivery expected until 7/4/2024 

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