Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Secondary |
Specific against |
other |
Amount |
500 ug |
Host |
Goat |
Item no. |
ABC-ASR2991 |
Conjugate/Tag |
HRP |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Bio Background |
F(ab')2 Anti-Mouse IgM mu heavy chain generated in goat detects specifically Mouse IgM mu heavy chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. |
Clonality |
Polyclonal |
Subtitle |
Goat Polyclonal, Peroxidase (Horseradish) |
Reactivity |
M |
Immunogen |
F(ab' )2 Anti-Mouse IgM mu heavy chain was produced by repeated immunization with Mouse IgM mu heavy chain in goat. |
Concentration (mg/ml) |
1.0 mg/mL by UV absorbance at 280 nm |
Conjugate |
Peroxidase (Horseradish) |
Application Note |
ELISA 1:100, 000; Western Blot 1:2, 000-1:10, 000; Immunochemistry 1:500-1:2, 500 |
Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Target Species |
Mouse |
Physical State |
Lyophilized |
Target Isotype |
IgM µ chain |
Host Isotype |
IgG F(ab')2 |
Reconstitution Volume |
500 µL |
Reconstitution Buffer |
Restore with deionized water (or equivalent) |
Stabilizer |
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative |
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Shipping Condition |
Ambient |
Purity |
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. |
Storage Condition |
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.