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F(ab')2 Anti-Rabbit IgG (H&L) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody

ArtNr ABC-ASR3170
Hersteller Abcepta
Menge 500 ug
Kategorie
Typ Antibody Secondary
Specific against other
Host Donkey
Konjugat/Tag AP
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Lieferbar
Bio Background
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Clonality
Polyclonal
Subtitle
Donkey Polyclonal, Alkaline Phosphatase (Calf Intestine)
Immunogen
Rabbit IgG whole molecule
Concentration (mg/ml)
1.0 mg/mL by UV absorbance at 280 nm
Conjugate
Alkaline Phosphatase (Calf Intestine)
Application Note
ELISA 1:4, 000-1:16, 000; Western Blot 1:500-1:2, 500; Immunohistochemistry 1:200-1:1, 000
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Target Species
Rabbit
Physical State
Liquid (sterile filtered)
Target Isotype
IgG (H&L)
Host Isotype
IgG F(ab')2
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% (w/v) Sodium Azide
Shipping Condition
Wet Ice
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Storage Condition
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 500 ug
Lieferbar: In stock
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