F(ab')2 Anti-Mouse IgG (H&L) (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody

F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated (Pre-Adsorbed) was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated (Pre-Adsorbed) is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Rabbit Polyclonal, R-Phycoerythrin (RPE)

Anti-Mosue IgG was produced by repeated immunization with Mouse IgG whole moleculein rabbit.

R-Phycoerythrin (RPE)

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Lyophilized

IgG F(ab')2

Restore with deionized water (or equivalent)

0.01% (w/v) Sodium Azide

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.  Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.  No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.