Hersteller |
Abcepta
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
other |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applikationen |
WB, FC, IHC-P |
Menge |
100 ul |
Host |
Rabbit |
ArtNr |
ABC-AW5245-U100 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Primary Accession |
P40925 |
Antigen Type |
Synthetic Peptide |
Application |
FC, IHC-P, WB |
Bio Background |
MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. |
Bio References |
Lee, S.M., et.al., Cell Death Differ. 16 (5), 738-748 (2009) |
Clonality |
Polyclonal |
Gene ID |
4190 |
Gene Name |
|
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of lysates from HL-60, THP-1 cell line (from left to right), using MDH1 Antibody (C-term)(Cat. #AW5245). AW5245 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:10~50 |
Legend image 3 |
MDH1 Antibody (C-term) (Cat. #AW5245) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 3 |
FC |
Dilution image 3 |
1:10~50 |
Other Accession |
O88989; P11708; P14152; Q5ZME2; Q3T145 |
Predicted |
B, C, M, Pig, Rat |
Isotype |
Rabbit Ig |
Calculated MW |
36426 |
Antigen Region |
286-314 |
Antigen Source |
HUMAN |
Target/Specificity |
This MDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-314 amino acids from the C-terminal region of human MDH1. |
NCBI Accession |
NP_001186040.1; NP_001186041.1; NP_005908.1 |
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