Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Primary |
Specific against |
other |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applications |
WB, FC, IHC-P |
Amount |
100 ul |
Host |
Rabbit |
Item no. |
ABC-AW5245-U100 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Primary Accession |
P40925 |
Antigen Type |
Synthetic Peptide |
Application |
FC, IHC-P, WB |
Bio Background |
MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. |
Bio References |
Lee, S.M., et.al., Cell Death Differ. 16 (5), 738-748 (2009) |
Clonality |
Polyclonal |
Gene ID |
4190 |
Gene Name |
|
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of lysates from HL-60, THP-1 cell line (from left to right), using MDH1 Antibody (C-term)(Cat. #AW5245). AW5245 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:10~50 |
Legend image 3 |
MDH1 Antibody (C-term) (Cat. #AW5245) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 3 |
FC |
Dilution image 3 |
1:10~50 |
Other Accession |
O88989; P11708; P14152; Q5ZME2; Q3T145 |
Predicted |
B, C, M, Pig, Rat |
Isotype |
Rabbit Ig |
Calculated MW |
36426 |
Antigen Region |
286-314 |
Antigen Source |
HUMAN |
Target/Specificity |
This MDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-314 amino acids from the C-terminal region of human MDH1. |
NCBI Accession |
NP_001186040.1; NP_001186041.1; NP_005908.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.