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Anti-CD146/MCAM Antibody Picoband® (monoclonal, 4C12)

Anti-CD146/MCAM Antibody Picoband® (monoclonal, 4C12)

ArtNr	BOS-M01683-3
Hersteller	Boster
Menge	100 ug/vial
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Kategorie	Neuroscience Neural Lineage Markers Neural Adhesion Molecules Antibodies Flow Cytometry Immunohistochemistry Western Blot Primary Antibodies Adhesion Molecule Markers
Typ	Antibody Monoclonal
Format	Lyophilized
Applikationen	WB, FC, IHC, IHC-F
Clon	4C12
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2a
Sensitivity	<10 pg/ml
Citations	1. Bardin, N., Anfosso, F., Masse, J.-M., Cramer, E., Sabatier, F., Le Bivic, A., Sampol, J., Dignat-George, F.Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion.Blood 98: 3677-3684, 2001. 2. Kuske, M. D. A., Johnson, J. P.Assignment of the human melanoma cell adhesion molecule gene (MCAM) to chromosome 11 band q23.3 by radiation hybrid mapping.Cytogenet. Cell Genet. 87: 258 only, 1999. 3. Taira, E., Nagino, T., Taniura, H., Takaha, N., Kim, C.-H., Kuo, C.-H., Li, B.-S., Higuchi, H., Miki, N.Expression and functional analysis of a novel isoform of gicerin, an immunoglobulin superfamily cell adhesion molecule.J. Biol. Chem. 270: 28681-28687, 1995.
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	A32 antigen antibody,CD 146 antibody,CD146 antibody,CD146 antigen antibody,Cell surface glycoprotein MUC18 antibody,Cell surface glycoprotein P1H12 antibody,Gicerin antibody,MCAM antibody,Melanoma adhesion molecule antibody,Melanoma associated antigen A32 antibody,Melanoma associated antigen MUC18 antibody,Melanoma associated glycoprotein MUC18 antibody,Melanoma cell adhesion molecule antibody,Melanoma-associated antigen A32 antibody,Melanoma-associated antigen MUC18 antibody,MelCAM antibody,MUC 18 antibody,MUC18 antibody,MUC18_HUMAN antibody,S endo 1 antibody,S endo 1 endothelial associated antigen antibody,S-endo 1 endothelial-associated antigen antibody,Sendo 1 endothelial associated antigen antibody,Sendo1 antibody
Lieferbar
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	120 kDa
Clonality	Monoclonal
Application Details	Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
Gene Name	MCAM
Gene Full Name	melanoma cell adhesion molecule
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.
Immunogen	E.coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.
Contents	Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction
Protein Name	activating transcription factor 1
Protein Function	Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Subcellular Localization	Membrane. Single-pass type I membrane protein.
Description	Boster Bio Anti-CD146/MCAM Antibody Picoband® (monoclonal, 4C12) catalog # M01683-3. Tested in Flow Cytometry, IHC, IHC-F, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.
Manufacturer - Alias	A32 antigen antibody, CD 146 antibody, CD146 antibody, CD146 antigen antibody, Cell surface glycoprotein MUC18 antibody, Cell surface glycoprotein P1H12 antibody, Gicerin antibody, MCAM antibody, Melanoma adhesion molecule antibody, Melanoma associated antigen A32 antibody, Melanoma associated antigen MUC18 antibody, Melanoma associated glycoprotein MUC18 antibody, Melanoma cell adhesion molecule antibody, Melanoma-associated antigen A32 antibody, Melanoma-associated antigen MUC18 antibody, MelCAM antibody, MUC 18 antibody, MUC18 antibody, MUC18_HUMAN antibody, S endo 1 antibody, S endo 1 endothelial associated antigen antibody, S-endo 1 endothelial-associated antigen antibody, Sendo 1 endothelial associated antigen antibody, Sendo1 antibody
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins.

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