POLA1 Antibody

POLA1

DKFZp686K1672 antibody; DNA polymerase alpha 1 catalytic subunit antibody; DNA polymerase alpha catalytic subunit antibody; DNA polymerase alpha catalytic subunit p180 antibody; DNA polymerase alpha p180 subunit antibody; DPOLA_HUMAN antibody; OTTHUMP00000023089 antibody; OTTHUMP00000023090 antibody; P180 antibody; p180 subunit antibody; POLA antibody; POLA1 antibody; Polymerase (DNA directed), alpha 1, catalytic subunit antibody; Polymerase (DNA-directed), alpha (70kD) antibody

Synthesized peptide derived from N-terminal of human DNA Polymerase alpha.

Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

Wong S.W., EMBO J. 7:37-47(1988).
Pearson B.E., Mol. Cell. Biol. 11:2081-2095(1991).
Hsi K.-L., Nucleic Acids Res. 18:6231-6237(1990).

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Epigenetics and Nuclear Signaling

Synthesized peptide derived from N-terminal of Human DNA Polymerase α.