ArtNr |
ICH1128UL-5MG |
Hersteller |
IchorBio
|
Menge |
5 mg |
Quantity options |
100 mg
25 mg
50 mg
5 mg
|
Kategorie |
|
Typ |
Antibody |
Applikationen |
WB, FC, IP, IHC, Blocking |
Clon |
PK136 |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Mouse |
Isotype |
IgG2a Kappa |
Purity |
>98% by SDS-PAGE and HPLC |
Citations |
Yang et al. Aconite aqueous extract inhibits the growth of hepatocellular carcinoma through CCL2-dependent enhancement of natural killer cell infiltration. Journal of Integrative Medicine |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Natural Killer Cells, KR-P1C, NKR-P1B, Ly-55, CD161b, CD161c |
Versandbedingung |
Gekühlt |
Lieferbar |
|
Specificity |
Anti-NK1.1 In Vivo Antibody - Ultra Low Endotoxin (PK136) recognizes an epitope on Mouse NK1.1 |
Manufacturer - Applications |
Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, CyTOF, IHC (Frozen) |
Manufacturer - Category |
Ultra-Low Endotoxin, anti-mouse, monoclonal antibody |
Manufacturer - Isotype |
Mouse IgG2a Kappa |
Manufacturer - Targets |
NK1.1 |
Shipping Temperature |
Blue ice |
Storage Conditions |
anti-NK1.1 In Vivo Antibody - Ultra Low Endotoxin (PK136) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
Aggregation |
Aggregation level ≤ 1% |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Antigen Distribution |
Mouse NK cells. Endogenous and interferon induced NK cells. Not present on cytotoxic T-cells and Con A stimulated blast cells. May be present on a certain subset of cytotoxic T-cells strains A, AKR, BALB/C, BALB.H-2b, CBA, C3H, DBA/2, 124 were negative using PK136. |
Application Notes |
Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. Each investigator should determine their own optimal working dilution for specific applications. |
Background |
NK-1.1 surface antigen, also known as CD161b/CD161c, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. |
Concentration |
≥ 2.0 mg/ml |
Endotoxin |
≤ 0.5 EU/mg as determined by the LAL method |
Manufacturer - Specificity |
Anti-NK1.1 In Vivo Antibody - Ultra Low Endotoxin (PK136) recognizes an epitope on Mouse NK1.1 |
Purification method |
This monoclonal antibody was purified using Protein A |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Immunogen |
NK-1+ cells from mouse spleen and bone marrow |
IMPACT Pathogen Test |
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp. Mycoplasma pulmonis Sendai virus Mouse hepatitis virus Pneumonia virus of mice Minute virus of mice Mouse parvovirus (MPV1-5) Theiler's murine encephalomyelitis virus Murine norovirus Reovirus 3 Mouse rotavirus Ectromelia virus Lymphocytic choriomeningitis virus Polyoma virus Lactate dehydrogenase-elevating virus Mouse adenovirus (MAD1, MAD2) Mouse cytomegalovirus K virus Mouse thymic virus Hantaan virus Corynebacterium bovis Corynebacterium spp. (HAC2) |
Related Antibodies |
|
Isotype Control |
Mouse IgG2a In Vivo Isotype Control - Ultra Low Endotoxin [C1.18.4] (ICH2245UL) |
Buffer |
ICH3001-100ml; ICH3002-100ml; ICH3003-100ml |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.