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Anti-Mouse NK1.1 In Vivo Antibody - Ultra Low Endotoxin (PK136)

Anti-Mouse NK1.1 In Vivo Antibody - Ultra Low Endotoxin (PK136)

Manufacturer	IchorBio
Category	Antibodies In Vivo Antibodies Flow Cytometry Western Blot Blocking
Type	Antibody
Specific against	Mouse
Clone	PK136
Isotype	IgG2a Kappa
Applications	WB, FC, IP, IHC, Blocking
Amount	5mg
Host	Mouse
Item no.	ICH1128UL-5MG
eClass 6.1	32160702
eClass 9.0	32160702
Available
Manufacturers Product Category	Ultra-Low Endotoxin, anti-mouse
Target Information	NK1.1
Isotype Information	Mouse IgG2a Kappa
Purification method	This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution	Mouse NK cells. Endogenous and interferon induced NK cells. Not present on cytotoxic T-cells and Con A stimulated blast cells. May be present on a certain subset of cytotoxic T-cells strains A, AKR, BALB/C, BALB.H-2b, CBA, C3H, DBA/2, 124 were negative using PK136.
Background	NK-1.1 surface antigen, also known as CD161b/CD161c, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.
Immunogen	NK-1+ cells from mouse spleen and bone marrow
Concentration	≥ 2.0 mg/ml
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Endotoxin	≤ 0.5 EU/mg as determined by the LAL method
Aggregation	Aggregation level ≤ 1%
IMPACT Pathogen Test	We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: - Mycoplasma spp. - Mycoplasma pulmonis - Sendai virus - Mouse hepatitis virus - Pneumonia virus of mice - Minute virus of mice - Mouse parvovirus (MPV1-5) - Theiler's murine encephalomyelitis virus - Murine norovirus - Reovirus 3 - Mouse rotavirus - Ectromelia virus - Lymphocytic choriomeningitis virus - Polyoma virus - Lactate dehydrogenase-elevating virus - Mouse adenovirus (MAD1, MAD2) - Mouse cytomegalovirus - K virus - Mouse thymic virus - Hantaan virus - Corynebacterium bovis - Corynebacterium spp. (HAC2)
Storage	anti-NK1.1 In Vivo Antibody - Ultra Low Endotoxin (PK136) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Application Notes	Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. - Each investigator should determine their own optimal working dilution for specific applications.
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 5mg

available

Delivery expected until 6/6/2024