ArtNr |
ICH1140-50MG |
Hersteller |
IchorBio
|
Menge |
50 mg |
Quantity options |
100 mg
1 mg
25 mg
50 mg
5 mg
|
Kategorie |
|
Typ |
Antibody |
Applikationen |
FC, IP, IV |
Clon |
UC3-10A6 |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Hamster - Armenian |
Isotype |
IgG |
Purity |
>95% by SDS-PAGE and HPLC |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
V gamma 2 |
Versandbedingung |
Gekühlt |
Lieferbar |
|
Manufacturer - Applications |
Flow Cytometry, Immunoprecipitation, In vivo Depletion |
Manufacturer - Category |
Low Endotoxin, anti-mouse, monoclonal antibody |
Manufacturer - Isotype |
Armenian Hamster IgG |
Manufacturer - Targets |
TCR V gamma 2 |
Shipping Temperature |
Blue ice |
Storage Conditions |
anti-TCR V gamma 2 In Vivo Antibody - Low Endotoxin (UC3-10A6) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
Aggregation |
Aggregation level ≤ 5% |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Antigen Distribution |
V gamma 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of gamma delta TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung. |
Application Notes |
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 µg per 1.0 X 106 cells in a 100 µl total staining volume, followed by Goat Goat Anti-Armenian Hamster IgG (H&L)-R-phycoerythrin Each investigator should determine their own optimal working dilution for specific applications. |
Background |
The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an alpha and beta chain in 95% of T cells, while 5% of T cells have TCRs consisting of gamma and delta chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules. |
Concentration |
1.0 - 5.0 mg/ml |
Endotoxin |
<1.0 EU/mg as determined by the LAL method |
Manufacturer - Specificity |
This antibody reacts with Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes. |
Purification method |
This monoclonal antibody was purified using Protein A |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Related Antibodies |
|
Isotype Control |
Armenian Hamster IgG Isotype Control for In Vivo - Low Endotoxin [PIP] (ICH2251) |
Buffer |
ICH3001-100ml; ICH3002-100ml; ICH3003-100ml |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.