Anti-Human IL-17 - 100 µg

CyTOF®, FC, ICC, IHC FF, IP, N, WB

IL-17

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Interleukin 17 (IL-17) is a T cell cytokine that induces monocyte migration and inflammation.1 IL-17-producing cell could serve as a potential prognostic marker and a novel therapeutic target for hepatocellular carcinoma (HCC).2 IL-17 may be important in recruiting monocytes into the joints of patients with RA, supporting IL-17 as a therapeutic target in RA.1

Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 2-15 µg/ml.
Immunoprecipitation: Suitable for use at concentration of 3 µg/100 µg cell lysate.
Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.
Neutralization:Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND₅₀) is typically 0.02-0.12 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.
CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Goat Anti-Human Interleukin-17 (IL-17) recognizes Human IL-17. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.