Mouse TNFα Antibody - Purified in vivo GOLD™ Functional Grade

Quality Tested by Leinco ELISA: Each lot of this antibody is quality control tested by ELISA assay. For use of this antibody as a capture, a concentration range of 2 - 6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF-α recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Tumor Necrosis Factor-Alpha

Next Day 2-8°C

The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity.1 It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.2 TNF-α is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-α secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-α has been detected in synovial fluid of patients with rheumatoid arthritis. Clone TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-α

N: Clone TN3-19.12 has been shown to neutralize murine TNFα
WB
IP
FC

Immunology, Innate Immunity

AB_2737571

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells

Recombinant mouse TNFα