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Mouse TNFα Antibody - Purified in vivo GOLD™ Functional Grade

Mouse TNFα Antibody - Purified in vivo GOLD™ Functional Grade

Item no.	LEIN-T258-5.0mg
Manufacturer	Leinco Technologies
Amount	5 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	WB, IP, IV
Clone	TN3-19.12
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1. El-Harith el-HA et al. (2004) Saudi Med J. 25: 1352. Adolf GR et al. (1990) Infec Immun. 58: 3996
NCBI	21926
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco ELISA: Each lot of this antibody is quality control tested by ELISA assay. For use of this antibody as a capture, a concentration range of 2 - 6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF-α recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Primary Monoclonal Antibodies>Growth Factors and Cytokines\|Purified in vivo Functional Grade
Manufacturer - Targets	Tumor Necrosis Factor-Alpha
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity.1 It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.2 TNF-α is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-α secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-α has been detected in synovial fluid of patients with rheumatoid arthritis. Clone TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-α
Background	The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity.¹ It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.² TNF-α is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-α secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-α has been detected in synovial fluid of patients with rheumatoid arthritis. Clone TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-α
Additional Reported Applications For Relevant Conjugates	N: Clone TN3-19.12 has been shown to neutralize murine TNFα WB IP FC
PubMed	Tumor Necrosis Factor-Alpha
Manufacturer - Research Area	Immunology, Innate Immunity
Manufacturer - Specificity	Armenian Hamster Anti-Mouse TNFa (Clone TN3-19.12 ) recognizes Mouse TNFa. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2737571
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	Recombinant mouse TNFα
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-T258-5.0mg-safety-datasheet.pdf