Anti-Human TIMP-1 (Clone 63515)

Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T416 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T411.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TIMP-1. A suggested method would be to stain cells expressing TIMP-1 with 0.25 µg per 1-5 x 10⁵ cells in a total staining volume of ≤200 µl followed by PN:M1189.
Western Blotting: To detect Human TIMP-1 this monoclonal antibody can be used at a concentration of 0.1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TIMP-1 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T411.

TIMP-1

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Tissue Inhibitor of Metalloproteinases 1, also known as TIMP1 is a widely expressed, secreted protein that functions primarily to inhibit members of a large family of metalloproteinases (MPs). Because of the ability of TIMP-1 to inhibit MPs, it functions in many of the same pathophysiological processes as these enzymes, e.g. wound healing, ovulation, angiogenesis, and cancer cell metastasis. TIMP-1 can also stimulate proliferation and cellular anabolic processes.1 TIMP-1 is a novel negative regulator of HGF activity during liver regeneration.2 Overexpression of TIMP-1 could promote renal interstitial fibrosis through the inflammatory pathway, which might be partly induced by upregulating ICAM-1.3

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10-15 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Immunocytochemistry: Suitable for use at concentration of 3-25 µg/mL.
CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Mouse Anti-Human Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) (Clone 63515) recognizes an epitope on Human TIMP-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Purified Recombinant Human TIMP-1 (Accession # P01033)