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Anti-Human TIMP-1 (Clone 63515) - 25 µg

Anti-Human TIMP-1 (Clone 63515) - 25 µg

Item no.	LEIN-T462-25ug
Manufacturer	Leinco Technologies
Amount	25 ug
Quantity options	100 ug 25 ug 500 ug
Category	Antibodies Flow Cytometry Immunohistochemistry Western Blot ELISA Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry
Type	Antibody Monoclonal
Applications	WB, FC, IHC, ICC, ELISA
Clone	63515
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Denhardt, DT.et al. (2005) Breast Cancer Res Treat. 94: 185 2. Khokha, R. et al. (2005) Hepatology 41: 857 3. Chen, X. et al. (2008) Nephrology Dialysis Transportation 23: 1861
NCBI	7076
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	CyTOF®, ELISA Cap, FC, ICC, IHC FFPE, WB
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	TIMP-1
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	Tissue Inhibitor of Metalloproteinases 1, also known as TIMP1 is a widely expressed, secreted protein that functions primarily to inhibit members of a large family of metalloproteinases (MPs). Because of the ability of TIMP-1 to inhibit MPs, it functions in many of the same pathophysiological processes as these enzymes, e.g. wound healing, ovulation, angiogenesis, and cancer cell metastasis. TIMP-1 can also stimulate proliferation and cellular anabolic processes.1 TIMP-1 is a novel negative regulator of HGF activity during liver regeneration.2 Overexpression of TIMP-1 could promote renal interstitial fibrosis through the inflammatory pathway, which might be partly induced by upregulating ICAM-1.3
Background	Tissue Inhibitor of Metalloproteinases 1, also known as TIMP1 is a widely expressed, secreted protein that functions primarily to inhibit members of a large family of metalloproteinases (MPs). Because of the ability of TIMP-1 to inhibit MPs, it functions in many of the same pathophysiological processes as these enzymes, e.g. wound healing, ovulation, angiogenesis, and cancer cell metastasis. TIMP-1 can also stimulate proliferation and cellular anabolic processes.¹ TIMP-1 is a novel negative regulator of HGF activity during liver regeneration.² Overexpression of TIMP-1 could promote renal interstitial fibrosis through the inflammatory pathway, which might be partly induced by upregulating ICAM-1.³
Other Applications Reported In Literature	IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 10-15 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Immunocytochemistry: Suitable for use at concentration of 3-25 µg/mL. CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
PubMed	TIMP-1
Manufacturer - Specificity	Mouse Anti-Human Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) (Clone 63515) recognizes an epitope on Human TIMP-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2831962
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human TIMP-1 (Accession # P01033)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Tissue Inhibitor of Metalloproteinases 1, EPO, CLGI, EPA, FLJ90373, HCI, TIMP, 63515, TIMP-1, Human TIMP-1, Anti-Human TIMP-1, CLGI, Collagenase inhibitor, collagenase inhibitor), EPATIMP-1, EPO, erythroid potentiating activity, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, FLJ90373, HCI, metalloproteinase inhibitor 1, TIMP metallopeptidase inhibitor 1, TIMP1, TIMP-1, TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, Tissue inhibitor of metalloproteinases 1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-T462-25ug-safety-datasheet.pdf