Vergleich

Human beta-Actin antibody

ArtNr NKM-ATGA0570-1ml
Hersteller NKMAX
Menge 1 ml
Quantity options 100 ug 1 ml 500 ul 50 ug
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, ICC, ELISA
Clon AT4G6
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Konjugat/Tag Unconjugated
Citations Gunning PW., et al. (2015) Journal of Cell Science. 128(11): 2009-2019.
Hanukoglu I., et al. (1983) Journal of Molecular Biology. 163(4): 673-8.
Yang YJ et al., Liquid Chromatography/Tandem Mass Spectrometry Analysis of Sophora flavescens Aiton and Protective Effects against Alcohol-Induced Liver Injury and Oxidative Stress in Mice. (Antioxidants (Basel). 2024)
Yang YJ et al., Korean Mistletoe (Viscum album var. coloratum) Ethanol Extracts Enhance Intestinal Barrier Function and Alleviate Inflammation. (Antioxidants (Basel) 2025)
Kim MJ et al., Potential Chondroprotective Effect of Artemisia annua L. Water Extract on SW1353 Cell. (Int. J. Mol. Sci. 2025)
Han JY et al., Cytoplasmic HMGB1 promotes the activation of JAK2-STAT3 signaling and PD-L1 expression in breast cancer. (Mol Med. 2025)
Kim MJ et al., Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition. (Sci Rep. 2025)
NCBI NP_001092
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Actin,cytoplasmic 1,Beta-actin
Similar products actin, beta actin, beta-actin, b-Actin, ATGA0457, ATGA0570-10ug, ATGA0570-20ug, ATGA0570-50ug, ATGA0570-100ug, ATGA0570-250ug, ATGA0570-500ug, ATGA0570-1mg, ATGA0570-10, ATGA0570-20, ATGA0570-50, ATGA0570-100, ATGA0570-250, ATGA0570-500, ATGA0570-1
Lieferbar
Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, WB, ICC/IF
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Concentration
0.2mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human ACTB (1-375aa) purified from E. coli
Ab Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-A affinity chromatography
Gene Category
Cancer
Manufacturer - Search Terms
b-Actin, beta actin, beta-actin, actin, ATGA0457, ATGA0570-10ug, ATGA0570-20ug, ATGA0570-50ug, ATGA0570-100ug, ATGA0570-250ug, ATGA0570-500ug, ATGA0570-1mg, ATGA0570-10, ATGA0570-20, ATGA0570-50, ATGA0570-100, ATGA0570-250, ATGA0570-500, ATGA0570-1
IF Comment
ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: PC3 cell lysate
Lane 4.: HepG2 cell lysate
Lane 5.: MCF7 cell lysate
Lane 6.: U87-MG cell lysate
Lane 7.: NIH-3T3 cell lysateThe mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Kidney tissue lysate
Lane 2.: Liver tissue lysate
Lane 3.: Spleen tissue lysate
Lane 4.: Bone marrow tissue lysate
Lane 5.: Stomach tissue lysate The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: ACTB antibody 1:1000
Lane 2.: ACTB antibody 1:3000
Lane 3.: ACTB antibody 1:5000
Lane 4.: ACTB antibody 1:10000

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 ml
Lieferbar: In stock
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