PUMA Antibody

Blue Ice

23 kDa

PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53 (1). The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (2). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Trial Size, IAV, KD, Cell Treatment, Most Cited

15193488

Human PUMA has 4 isoforms, including isoform 1 (193aa, 21kD), isoform 2 (131aa, 14kD), isoform 3 (101aa, 10kD) and isofrom 4 (261aa, 27kD). This antibody detects human isoform 1&2, mouse and rat PUMA (193aa, 21kD for both of them).

PRS-1210-0.1mg

PRS-1220-0.1mg

1 mg/mL

WB: 1-4 μ g/mL; IF: 2 μ g/mL; ICC: 1 μ g/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.

A lower band at approximately 16 kDa was detected in Daudi and K562 cells, which may represent the PUMA isoform 2.

Polyclonal

Homo sapiens

Optimal dilutions for each application to be determined by the researcher.