ACTN4 Antibody

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50

blue ice or room temperature

105 kDa

Alpha actinins belong to the spectrin superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This protein is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and is thought to be involved in metastatic processes. Mutations in the gene encoding this protein have been associated with focal and segmental glomerulosclerosis.

Batch dependent

This ACTN4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 785-813 amino acids from the C-terminal region of human ACTN4.

Alpha-actinin-4

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.