Neurturin Antibody

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hNeurturin (50.00 ng/mL), a concentration of 0.5 - 0.75 μg/mL of this antibody is required.

ELISA:

To detect hNeurturin by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNeurturin.

Sandwich:

To detect hNeurturin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Neurturin (38-115) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNeurturin.

Western Blot:

To detect hNeurturin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNeurturin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Neurturin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Human Neurturin (NTN) and Human GDNF comprise a family of TGF-beta related neurotrophic factor which have trophic influences on a variety of neuronal populations. Neurturin promotes the survival of certain sympathetic and sensory neurins through interaction with distinct set of GDNF-like receptors

Anti-hNeurturin specific antibody was purified by affinity chromatography employing immobilized hNeurturin matrix.

Polyclonal

Homo sapiens

Centrifuge vial prior to opening.