Anti-GABPB2 antibody (Internal) (STJ71191)

Pep-ELISA/WB/IHC/IF/FC

Store at-20°C on receipt and minimise freeze-thaw cycles.

0.5 mg/mL

Polyclonal

Peptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 45+38kDa bands observed in lysates of cell line HeLa (calculated MW of 42.5kDa according to NP_005245.2, NP_001307844.1 and of 38.1kDa according to NP_002032.2). An additional band of unknown identity was also consistently observed at 22kDa and was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Tonsil and Spleen. Recommended concentration: 3.75µg/ml.
IF: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Internal