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Rabbit anti-PSMA2 Antibody

ArtNr YLD6466-100ul
Hersteller YL Biotech
Menge 100ul
Kategorie
Typ Antibody Polyclonal
Applikationen WB, IF, IHC, IC
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias HC3; PSC3; Proteasome subunit alpha type-2; Macropain subunit C3; Multicatalytic endopeptidase complex subunit C3; Proteasome component C3
Lieferbar
Background
Rabbit polyclonal antibody to PSMA2
Concentration
1mg/mL
Immunogen
Recombinant full length protein of human PSMA2
WB description
Western blot analysis of PSMA2 expression in Jurkat (A), Hela (B), NIH3T3 (C), mouse brain (D) whole cell lysates.
IHC description
Immunohistochemical analysis of PSMA2 staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of PSMA2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Purification_Method
The antibody was purified by immunogen affinity chromatography.
Clonality
Polyclonal
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Storage
Store at -20℃. Avoid repeated freeze / thaw cycles.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100ul
Lieferbar: In stock
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