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Human Glycosylation Array L4

Human Glycosylation Array L4

Hersteller	Raybiotech
Kategorie	Arrays Protein Arrays
Typ	Array
Specific against	other
Menge	4 Sample Kit
Host	Hamster - Armenian
ArtNr	GAH-GCM-L4-4
eClass 6.1	32161000
eClass 9.0	32161000
Lieferbar
Number of Targets Detected	500
Species Detected	Human
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Plasma Serum Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Fluorescence Laser Scanner
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Glass Slide
Gene Symbols	ABCC1 \| ACP1 \| ACTG1 \| AFF3 \| AGRN \| AMY1A \| ANP32A \| AOC3 \| AQR \| ARHGDIA\| ARPC5 \| BCAS1 \| CADM1 \| CADM3 \| CCAR2 \| CCDC80 \| CCS \| CCT7 \| CDH2 \| CDHR5 \| CDK5R2 \| CFP \| DLD \| DNPH1 \| DYNLL1 \| EIF6 \| ERN1 \| ERP44 \| F11R \| FERMT3 \| FUS \| GRM5 \| GUCA2B \| HSPA1A \| HTRA1 \| HYOU1 \| IDH1 \| IDH3A \| IFRD1 \| IGF2BP2 \| IGFBP7 \| IHH \| ILK \| INHBE \| INPP5D \| IQGAP1 \| IQGAP2 \| IRS2 \| ISOC2 \| ITGA6 \| ITGB1 \| ITGB6 \| ITIH1 \| ITIH2 \| ITIH3 \| ITIH4 \| JARID2 \| KCNMA1 \| KDM4B \| KHSRP \| KIAA0319L \| KIAA1468 \| KIF5B \| KLKB1 \| KMT2D \| KPNB1 \| KRT31 \| KRT33B \| KRT36 \| KRT38 \| KRT72 \| KRT73 \| KRT82 \| KRT85 \| KRTDAP \| KSR1 \| LAIR1 \| LAMA1 \| LAMA2 \| LAMB1 \| LAMB2 \| LAMC1 \| LAMP1 \| LAMP2 \| LAMTOR3 \| LAP3 \| LASP1 \| LCAT \| LCMT2 \| LCP1 \| LDHA \| LDHB \| LEMD3 \| LILRA3 \| LIMS1 \| LMAN2 \| LMNA \| LMNB1 \| LMNB2 \| LOX \| LOXL1 \| LRP4 \| LSAMP \| LTA4H \| LTBP2 \| LTBP4 \| LUZP1 \| LYPLA1 \| LYZ \| MAGI2 \| MAN2A1 \| MANF \| MAP1A \| MAPRE1 \| MARCKS \| MASP1 \| MBD2 \| MBP \| MCAM \| MCFD2 \| MCL1 \| MCM2 \| MCM5 \| MDH1 \| MDH2 \| ME1 \| MED1 \| MELTF \| MEP1A \| MFAP4 \| MFAP5 \| MGP \| MINPP1 \| MMRN1 \| MMRN2 \| MN1 \| MPO \| MRC1 \| MSH6 \| MSN \| MT2A \| MTOR \| MTPN \| MUT \| MYBPC3 \| MYDGF \| MYH1 \| MYH2 \| MYH6 \| MYH7 \| MYH9 \| MYL12B \| MYL3 \| MYLK \| MYO18B \| MYO5A \| MYOF \| NAGLU \| NAP1L1 \| NAPRT \| NASP \| NCAM2 \| NEB \| NECTIN1 \| NECTIN3 \| NEDD8 \| NEFM \| NEGR1 \| NEO1 \| NF1 \| NID2 \| NIT2 \| NME3 \| NOS1 \| NOTCH2 \| NPAS3 \| NPB \| NPM1 \| NQO2 \| NRGN \| NRP1 \| NT5C3A \| NTM \| NUCB1 \| NUP98 \| OGN \| OIT3 \| OLFM1 \| OLFM2 \| OMD \| OPCML \| ORM2 \| OSBP \| OSCAR \| OSMR \| OTC \| OXT \| P4HB \| PA2G4 \| PABPC1 \| PACS1 \| PARVB \| PCBP1 \| PCBP2 \| PCCA \| PCDH7 \| PCDH8 \| PCK2 \| PCMT1 \| PCNA \| PCOLCE \| PCSK9 \| PCYOX1 \| PDE1B \| PDIA6 \| PDLIM1 \| PDLIM5 \| PDZD2 \| PEBP1 \| PEBP4 \| PEPD \| PER1 \| PF4V1 \| PFN1 \| PGAM1 \| PGAM2 \| PGD \| PGK1 \| PGLS \| PGLYRP2 \| PGM1 \| PHGDH \| PIGR \| PIK3C2B \| PIK3IP1 \| PISD \| PKLR \| PKP1 \| PLA2G1B \| PLCG1 \| PLEC \| PLEK \| PLIN1 \| PLIN3 \| PLOD1 \| PLOD2 \| PLS3 \| PLXDC2 \| PLXNB1 \| PLXNB2 \| PNP \| POLR2A \| POR \| POSTN \| PPCS \| PPOX \| PPP1R9A \| PPP2R1B \| PPP2R4 \| PRCP \| PRDM13 \| PRDX1 \| PRDX2 \| PRDX3 \| PRDX5 \| PRELP \| PREP \| PRG2 \| PRG4 \| PRNP \| PROC \| PROZ \| PRSS2 \| PRSS23 \| PRSS3 \| PRTN3 \| PSAP \| PSAT1 \| PSIP1 \| PSMA1 \| PSMA2 \| PSMA3 \| PSMA4 \| PSMA5 \| PSMA6 \| PSMA7 \| PSMB1 \| PSMB2 \| PSMB3 \| PSMB4 \| PSMB5 \| PSMB6 \| PSMB7 \| PSMC3 \| PSMD1 \| PSMD5 \| PSMD9 \| PTEN \| PTGDS \| PTGES3 \| PTK7 \| PTMA \| PTPN6 \| PTPRG \| PTPRK \| PTPRM \| PTPRS \| PTPRZ1 \| PYGL \| PZP \| QDPR \| QPRT \| QSOX1 \| RAB7A \| RAN \| RANGAP1 \| RAP1B \| RBM15 \| REG1A \| REG3A \| RHOC \| RNASE1 \| RNASE4 \| RNASE6 \| RNASET2 \| RNH1 \| RPL10 \| RPL10A \| RPL11 \| RPL12 \| RPL14 \| RPL17 \| RPL22 \| RPL5 \| RPL7A \| RPLP0 \| RPS10 \| RPS11 \| RPS12 \| RPS19 \| RPS2 \| RPS20 \| RPS23 \| RPS25 \| RPS28 \| RPS3 \| RPS5 \| RREB1 \| RSU1 \| S100A1 \| S100A11 \| S100A7 \| S100A9 \| S100P \| SAA \| SAA4 \| SAMSN1 \| SARS \| SBSN \| SCARF2 \| SCG5 \| SCGB1A1 \| SCP2 \| SDF4 \| SDPR \| SELENBP1 \| SEMA6B \| SEMA7A \| SEMG1 \| SEMG2 \| SERPINA10 \| SERPINA11 \| SERPINA7 \| SERPINB1 \| SERPINB3 \| SERPINB4 \| SERPINB6 \| SERPINB8 \| SERPINE2 \| SERPINF2 \| SET \| SEZ6L2 \| SFTPD \| SH3BGRL \| SH3BGRL3 \| SHANK1 \| SHC1 \| SHMT1 \| SIGLEC1 \| SIGLEC14 \| SIM2 \| SIRPB1 \| SIX3 \| SLC38A10 \| SLITRK1 \| SLURP1 \| SMC4 \| SMPD4 \| SOD1 \| SOD2 \| SOD3 \| SORD \| SORL1 \| SOX4 \| SPEN \| SPINK5 \| SPINK7 \| SPTBN1 \| SPTBN5 \| SRC \| SST \| STAT3 \| STIP1 \| STK10 \| STMN1 \| STOM \| STX7 \| SUCLG1 \| SUMO3 \| SYMPK \| SYNE2 \| TAGLN2 \| TALDO1 \| TAX1BP3 \| TBCA \| TCEB2 \| TCF20 \| TCN1 \| TERF2 \| TFF2 \| TGM3 \| THOP1 \| TKT \| TLN1 \| TLN2 \| TMEM223 \| TMSB10 \| TNC \| TNKS1BP1 \| TNXB \| TOB2 \| TOP2B \| TP73 \| TPI1 \| TPM3 \| TPM4 \| TPP1 \| TRAP1 \| TRPS1 \| TSR2 \| TTN \| TTR \| TUBA1C \| TWF2 \| TWIST1 \| TXN \| TXNDC5 \| TXNRD2 \| UBE2D3 \| UBE2L3 \| UBE2N \| UCHL1 \| UFM1 \| UGGT1 \| UNC13D \| UNC45A \| UNC5D \| UPB1 \| UQCRB \| UQCRH \| URB2 \| UROC1 \| UROD \| USP14 \| USP5 \| UTRN \| VAPA \| VCAN \| VCL \| VCP \| VDAC1 \| VIM \| VNN1 \| VSIG4 \| WARS \| WDR1 \| WISP2 \| WNK2 \| YBX1 \| YY1 \| ZBTB4 \| ZC3H4 \| ZC3H8 \| ZDHHC18 \| ZNF671 \| ZYX
Application Information	Identify and profile the glycans in their samples Determine whether their biomarker of interest has glycan moieties Determine relative expression levels
Kit Components	Human Glycosylation Array L4 Glass Slide/s Dilution Buffer20 X Wash Buffer I20 X Wash Buffer I I Cy3 equivalent dye-conjugated Streptavidin Biotin-labeled Lectin Mixture Adhesive device sealer Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Distilled or de-ionized water1 ml microcentrifuge tubes Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline	Dry the glass slide Incubate samples Incubate with biotin lectin mixture Incubate with Cy3 Equivalent Dye- Streptavidin Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
Product Features	High density screening High sensitivity and specificity Low sample volume (20-100 µl per array) Large dynamic range of detection Compatible with most sample types Accurate and reproducible
Introduction	Recent technological advances by RayBiotech have enabled the largest commercially available glycosylation antibody array to date. With the Human Glycosylation Array L4, researchers can now efficiently obtain glycosylation profiles in their samples. The glycosylation profiles of 500 human proteins can be simultaneously detected, including, but not limited to, cytokines, chemokines, adipokines, growth factors, angiogenic factors, proteases, soluble receptors, soluble adhesion molecules in cell culture supernatants, serum, plasma and other samples types.Capture antibodies are printed onto glass slides and the glycans on these capture antibodies are removed. The glass slide arrays come pre-blocked and are ready to be incubated with samples. After incubation with samples and washing to remove unbound proteins, five unique biotin-labeled lectins are incubated with the array to allow binding to the glycans from captured target proteins. Streptavidin-conjugated fluorescent dye (Cy3 equivalent) is then added to the array. Finally, the glass slide is dried and laser fluorescence scanning is used to visualize the signals. These signals are then compared to the array map to identify glycosylated proteins present in the samples.
Typical Results	The following figure shows the RayBio® Human Glycosylation Antibody Array L4 probed with conditioned media sample. The images were captured using a Axon GenePix laser scanner. The strong signals in row 20 and the upper left and lower right corners of each array are Positive Controls, which can be used to identify the orientation and help normalize the results between arrays.
Biotin-Labeled Lectin Mixture	Lectin Name Sugar specificity

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