Human Glycosylation Array L4

Identify and profile the glycans in their samples
Determine whether their biomarker of interest has glycan moieties
Determine relative expression levels

mTOR, PTEN, Ubiquitin+1, Complement MASP3, HSPA1A, HTRA1, Human Agrin, IBP160, IDH1, IDH3A, IFRD1, Ihh, ILK, IMP2, Inhibin beta, Integrin alpha-6, IQGAP1, IQGAP2, IRE1, IRS2, ISOC2, ITGB4BP, ITIH1, ITIH2, ITIH3, ITIH4 a, JARID2, Karyopherin beta 1, Keratin 36, Keratin 38, KHSRP, KIAA0319L, KIAA1468, KIAA1967, KIF5B, Kilon, KLK-B1, KMD4B, KMT2B, KRT31, KRT72, KRT73, KRT82, KRT85 N-terminal, KRTDAP, KRTHA3B, KSR1, LAD, LAF4, LAIR1, LAM b1, LAMA, Lamin A/C, Lamin B1, Lamin B2, Laminin 2 alpha, Laminin b2, Laminin gamma 1, LAP3, LASP1, Latent TGF beta bp2, LCAT, LCMT2, LDHA, LDHB, LEDGF, LILRA3, LIMS1, LMAN2, LOK, LOX, LOXL1, LRP 4, LTA4H, LTBP4, Lubricin, LUZP1, LYPA1, Lysozyme, MAGI2, MAN1, MANF, Mannosidase II, MAP1A, MAPRE1, MARCKS, MBD2, MBP, MCM, MCM5, MCMP2, MDH1, MDH2, ME1, Meprin alpha, Metallothionein, Metavinculin, MFAP4, MFI2, mGLUR5, MGP, MINPP1, MLCK, MMRN1, MN1, Moesin, MP1, MPCA, MRP 1, MSH6, MUCDHL, Multimerin 2, MyBPC3, Myeloperoxidase, MYH2, MYH6, MYH7, MYHC, MYL12B, MYL3, MYO5A, Myoferlin, Myosin 18B, Myotrophin, NABC1, NAGLU, NAP1L1, NAPRT1, NASP, NCAM2, Nebulin, Nectin-1, Nectin-3, NEDD8, Neogenin, Nesprin2, Neurabin 1, Neurofibromin, Neurogranin, Neuropeptide B, Neuropilin-1, Neurotrimin, NF-M, Nidogen-2, NIT2, NME3, nNOS, Noelin, Non-muscle Actin, Non-muscle Myosin IIA, Notch-2, Notch-2 ICD, NPAS3, NPM1, NQO2, NT5C3, Nucleobindin 1, NUP98, OIT3, Ornithine Carbamoyltransferase, Orosomucoid 2, ORP150, OSBP1, OSCAR, Oxytocin-neurophysin 1, p16 ARC, P20Sb3, p23, p39, P4HB, p73, PA2G4, PABP, PACS1, PARVB, PCBP1, PCBP2, PCCA, PCDH7, PCDX8, PCK2, PCMT1, PCNA, PCPE-1, PCYOX1, PDE1B, PDIA6, PDLIM1, PDLIM5, PDZD2, PEBP4, PEPD, PER1, Perilipin-1, Perilipin-3, Periostin, Peroxiredoxin 2, Peroxiredoxin 3, Peroxiredoxin 5, PF4V1, PGAM1, PGAM2, PGD, PGDF, PGK-1, PGLS-C-t, PGM1, PGRPL, PHAP1, Phosphoserine Aminotransferase, PI3-Kinase��C2 beta, pIgR, PIK3IP1, PIN, PISD, PKLR, PLA2G1B, Plakophilin 1, Plastin L, PLC-gamma 1, Pleckstrin, Plectin, Plexin B1, Plexin B2, PLOD1, PLOD2, PLS3, Plxdc2, PNP, POR, PPCS, PPOX, PPP2R1B, PPP2R4, PRCP, PRDM13, PRDX 1, PRELP, PREP, PRG2, Prion protein PrP, Profilin 1, Properdin, Prosaposin, Prostaglandin D Synthase, Proteasome 20S a+b, Proteasome 20S alpha, Proteasome 20S alpha 5, Proteasome 20S b7, Proteasome 26S S5, Proteasome beta 1, Proteasome subunit alpha type 6, Proteasome subunit beta type 2, Proteasome subunit beta type 4, Protein Z, Prouroguanylin, PRSS23, PSMA1, PSMA2, PSMA4, PSMA7, PSMB5, PSMC3, PSMD1, PSMD9, PTK 7, PTMA, PTP gamma, PTP kappa, PTP mu, PTPRS, PTPRZ, PYGL, PZP, QDPR, QPRT, Quiescin Q6, Rab7a, Ran, RanGAP1, RAP1AB, Rbm15, RCL, Reg1A, Reg3A, RHOC, RhoGDI, Ribonuclease A, Ribonuclease Inhibitor, Ribonuclease T2, RKIP, RNA Polymerase��II, RNASE4, RNASE6, RPL10, RPL10A, RPL11, RPL12, RPL14, RPL17, RPL22, RPL5, RPL7A, RPLP0, RPS10, RPS11, RPS12, RPS19, RPS2, RPS20, RPS23, RPS25, RPS28, RPS3, RPS5, RREB1, RSU1, S100 A1, S100 A11, S100 A7, S100 A9, S100P, SAA4a, Salivary alpha amylase, SAMSN1, SBP-1, SBSN, SDF4, SDNSF, SDPR, Secretogranin V, Semaphorin 6B, Semaphorin 7A, Semenogelin I, Semenogelin II, Serpin A11, Serpin A7, Serpin B1, Serpin B8, Serpin E2, Serpin F2, Serpin��A10, Serpin��B6, SerRS, SET, SEZ6L2, SF20, SH3BGRL, SH3BGRL3, SHANK1, SHC1, SHIP, SHMT1, SHP-1, Siglec-14, SIM2, SIRP beta 1, Six3, SLC38A10, SLITRK1, SLURP1, SMA, SMC4, SMPD4, SOD1, SOD2, SOD3, SOD4, Somatostatin, SORD, SorLA, SOX4, SP-D, Spectrin beta-5, SPEN, SPINK7, SPTBN1, Src(1), STAT3, Stathmin 1, Sterol carrier protein 2, STI1, STOM, SUCLG1, SUMO3, Symplekin, SynCAM, Syntaxin 7, TAB182, TAGLN2, Talin1, Talin1&2, TAX1BP3, TBCA, TCEB2, Tcf20, TCN1, TCP1 eta, Tenascin X(1), Tenascin��C, TFF2(1), TGM3, Thimet��Oligopeptidase, Thioredoxin-1, Thymosin b10, Titin, TLS, TMEM223, TOB2, TOP2B, TPM4, Transketolase, Transthyretin, TRAP1, TRAP220, TRF 2, Triosephosphate isomerase, Tropomyosin 3, TRP-1, TRPS1, Trypsinogeb-2, TrypsinPan, Tryptophanyl, TSR2, TUBA6, TWF2, TXNDC4, TXNDC5, TXNRD2, UBE2D3, Ube2L3, UBE2N, UCH-L1, UFM 1, UGGT, UNC-13 Homolog D, UNC45A, UNC5H4, UPB1, UQCRB, UQCRH, URB, URB2, UROC1, UROD, URP2, USP14, USP5, Utrophin, VAP-A, VCP, VDAC1, Versican isoform V0, Vimentin B, VNN1, VSIG4, WDR1, WISP2, WNK2, YB1, YY1, ZBTB4, ZC3H4-N-t, ZC3H8, ZDHHC18, ZNF671, Zyxin, Integrin beta-6, Mcl-1 / Bcl2L3, LMW-PTP / ACP-1, N-Cadherin / Cadherin-2, Integrin beta-1, LEKTI / SPINK5, Uteroglobin / SCGB1A1, IGSF4B / SynCAM3 / CADM3, Coagulation Factor XIV / Protein C / F14, LAMP1 / CD107a, LAMP2 / CD107b, MMR / CD206 / MRC1, Proprotein Convertase 9 / PCSK9, SAA / Serum Amyloid A, JAM-A / CD321 / F11R, IGFBP-rp1 / IGFBP-7, MAGP-2 / MFAP5, MCAM / CD146, OBCAM / OPCML, Olfactomedin-2 / Noelin-2, Osteoadherin / Osteomodulin, Oncostatin M R beta / IL-31 R beta, Proteinase 3 / Myeloblastin, SCCA2 / Serpin B4, Serpin B3 / SCCA1, Siglec-1 / CD169, SREC-II / SCARF2, Tripeptidyl-peptidase I / TPP1, Trypsin 3 / PRSS3, VAP-1 / AOC3, LAMP / LSAMP, Mimecan / Osteoglycin, Transaldolase 1 / TALDO1

-20°C

RayBio® Human Glycosylation Antibody Array L4. For the simultaneous detection of the glycosylation protein profile of 500 human proteins in serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.

Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates

Fluorescence Laser Scanner

Glass Slide

Human Glycosylation Array L4 Glass Slide/s
Dilution Buffer20
X Wash Buffer I20
X Wash Buffer IICy3 equivalent dye-conjugated Streptavidin
Biotin-labeled Lectin Mixture
Adhesive device sealer
Manual

Dry the glass slide
Incubate samples
Incubate with biotin lectin mixture
Incubate with Cy3 Equivalent Dye-Streptavidin
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

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The following figure shows the RayBio® Human Glycosylation Antibody Array L4 probed with conditioned media sample. The images were captured using a Axon GenePix laser scanner. The strong signals in row 20 and the upper left and lower right corners of each array are Positive Controls, which can be used to identify the orientation and help normalize the results between arrays.