Munc-18 Antibody (Phospho-Ser515)

Polyclonal Antibody

Wet Ice

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Immunostaining of 14 DIV cortical neurons showing specific labeling of Munc-18 phosphorylated at Ser515 (red) and PSD95 (green). Photo courtesy of Gang Liu.
Western blot of rat cortex lysate showing specific immunolabeling of the ~65k Munc-18 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).

Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc-18 is thought to be regulated by PKC phosphorylation of Ser515 on the Munc-18 protein (Sassa et al., 1996).

Syntaxin-binding protein 2

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Cow; Dog; Human; Mouse; Rat