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STK39 Chemi-Luminescent ELISA Kit (Human)

ArtNr OKCD03930
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen CLIA, IA
Specific against Human (Homo sapiens)
Sensitivity 0.78 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias DCHT;PASK;proline-alanine-rich STE20-related kinase;serine threonine kinase 39 (STE20/SPS1 homolog,yeast);serine/threonine-protein kinase 39;small intestine SPAK-like kinase;SPAK;STE20/SPS1 homolog;STE20/SPS1-related proline-alanine-rich protein kinase;Ste20-like protein kinase;ste-20-related kinase.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
STK39
Gene Fullname
serine/threonine kinase 39
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
May act as a mediator of stress-activated signals. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation.
Nucleotide accession_num
NM_013233
Protein accession_num
NP_037365
Protein name
STE20/SPS1-related proline-alanine-rich protein kinase
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
STK39 Microplate: 96 Wells (12 x 8 Well strips)
STK39 Lyophilized Standard: 2
100X STK39 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Serine/Threonine Kinase 39 (STK39). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Serine/Threonine Kinase 39 (STK39). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Serine/Threonine Kinase 39 (STK39) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine/Threonine Kinase 39 (STK39) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine/Threonine Kinase 39 (STK39) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Tissue homogenates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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