Comparison

STK39 Chemi-Luminescent ELISA Kit (Human)

Item no. OKCD03930
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Applications CLIA, IA
Specific against Human (Homo sapiens)
Sensitivity 0.78 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias DCHT;PASK;proline-alanine-rich STE20-related kinase;serine threonine kinase 39 (STE20/SPS1 homolog,yeast);serine/threonine-protein kinase 39;small intestine SPAK-like kinase;SPAK;STE20/SPS1 homolog;STE20/SPS1-related proline-alanine-rich protein kinase;Ste20-like protein kinase;ste-20-related kinase.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
STK39
Gene Fullname
serine/threonine kinase 39
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
May act as a mediator of stress-activated signals. Mediates the inhibition of SLC4A4, SLC26A6 as well as CFTR activities by the WNK scaffolds, probably through phosphorylation.
Nucleotide accession_num
NM_013233
Protein accession_num
NP_037365
Protein name
STE20/SPS1-related proline-alanine-rich protein kinase
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
STK39 Microplate: 96 Wells (12 x 8 Well strips)
STK39 Lyophilized Standard: 2
100X STK39 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Serine/Threonine Kinase 39 (STK39). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Serine/Threonine Kinase 39 (STK39). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Serine/Threonine Kinase 39 (STK39) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine/Threonine Kinase 39 (STK39) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine/Threonine Kinase 39 (STK39) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Tissue homogenates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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