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QuantiChrom Acetylcholinesterase Assay Kit

Hersteller BioAssay Systems
Kategorie
Typ Assay-Kit
Specific against other
Menge 100 Tests
ArtNr BAS-DACE-100
Targets ACHE ;; PON1
eClass 6.1 32161000
eClass 9.0 32161090
Lieferbar
Description
Quantitative determination of acetylcholinesterase activity by colorimetric (412nm) method. Procedure: 10 min. Kit size: 100 tests. Detection limit: 10 U/L. Shelf life: 6 months. Shipping: ambient temp; storage: room temp.
Faqs

Does the assay detect only acetylcholinesterase activty?

No, this assay detects acetylcholinesterase and butyrylcholinesterase (also known as plasma cholinesterase, pseudocholinesterase) and does not distinguish between the two.

Which anticoagulant should I use for blood sample collection?

We recommend using serum for measuring plasma cholinesterase activity and EDTA blood for measuring red blood cell acetylcholinesterase activity.


For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Overview

Application

  • For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors.

Key Features

  • Sensitive and accurate. Detection range 10 to 600 U/L AChE activity in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the optical density at 2 min and 10 min at room temperature.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

  • OD412nm

Samples

  • Blood, serum, plasma etc

Species

  • All

Size

  • 100 tests

Detection Limit

  • 10 U/L

Shelf Life

  • 6 months

More Details

  • ACETYLCHOLINESTERASE (EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. Low serum cholinesterase activity may relate to exposure to insecticides or to one of a number of variant genotypes. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Cholinesterase levels of cells and plasma are used as a guide in establishing safety precautions relative to exposure and contact, as well as a guide in determining the need for workers to be removed from areas of contact with the organic phosphate insecticides. Simple, direct and automation-ready procedures for measuring AChE activity are very desirable. BioAssay Systems QuantiChrom Acetylcholinesterase Assay is based on an improved Ellman method, in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5, 5’-dithiobis(2-nitrobenzoic acid). The intensity of the product color, measured at 412 nm, is proportionate to the enzyme activity in the sample.
Shipment
RT
Storage
-20 C

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 Tests
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 25.04.2024 

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