QuantiChrom alpha-Glucosidase Assay Kit

Application

• For quantitative determination of ?-glucosidase activity and evaluation of drug effects on its metabolism.

Key Features

• High sensitivity and wide linear range. Use 20 ?L sample. The detection limit is 2 U/L, linear up to 250 U/L.
• Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ?-glucosidase activity within 20 minutes.
• Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

Method

• OD405nm

Samples

• Biological

Species

• All

Size

• 100 tests

Detection Limit

• 2 U/L

Shelf Life

• 6 months

More Details

• ?-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1, 4-linked ?-D-glucose residues with release of ?-D-glucose. ?-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct and automation-ready procedures for measuring ?-glucosidase activity are becoming popular in Research and Drug Discovery. BioAssay Systems QuantiChrom ?-Glucosidase Assay Kit is designed to measure ?-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-?-D-glucopyranoside that is hydrolyzed specifically by ?-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.